Experiments in Biochemistry

Course Information

College	Toyama College	Year	2022
Course Title	Experiments in Biochemistry
Course Code	0097	Course Category	Specialized / Compulsory
Class Format	Experiment / Practical training	Credits	Academic Credit: 2
Department	Department of Applied Chemistry and Chemical Engineering	Student Grade	4th
Term	First Semester	Classes per Week	2
Textbook and/or Teaching Materials
Instructor	Goto Michimasa,Shinozaki Yukiko

Course Objectives

At the completion of this course, students will be able to

1) Can perform antibacterial activity test by paper disk method and assess the results
2) Can perform quantification of protein by Lowry method and understand the quantitative theory
3) Can perform enzyme kinetics experiment with β-galactosidase and calculate the reaction velocity parameters(Km and Vmax)
4) Can calculate pKa by measurement of pH of amino acid solution and understand what is the pKa
5) Can immobilize beaker’s yeast, perform the experiment on alcohol fermentation, and quantify the glucose

Rubric

	Ideal Level of Achievement	Standard Level of Achievement	Unacceptable Level of Achievement)
Evaluation 1	Can perform antibacterial activity test by paper disk method and assess the results almost perfectly	Can perform antibacterial activity tests with the paper disk method and assess the results correctly	Can't perform antibacterial activity tests with the paper disk method and assess the results correctly
Evaluation 2	Can perform quantification of protein by Lowry method accurately and understand the quantitative theory almost perfectly	Can perform quantification of protein using the Lowry method and understand the quantitative theory correctly	Can't perform quantification of protein using the Lowry method and understand the quantitative theory correctly
Evaluation 3	Can perform enzyme kinetics experiment with β-galactosidase and calculate the reaction velocity parameters(Km and Vmax) almost perfectly	Can perform enzyme kinetics experiment with β- galactosidase and calculate the reaction velocity parameters (Km and Vmax) correctly	Can't perform enzyme kinetics experiment with β- galactosidase and calculate the reaction velocity parameters (Km and Vmax) correctly
Evaluation 4	Can calculate pKa accurately by measurement of pH of amino acid solution and understand what is the pKa almost perfectly	Can calculate pKa accurately by measurement of pH of amino acid solution and understand what is the pKa almost correctly	Can't calculate pKa accurately by measurement of pH of amino acid solution and understand what is the pKa almost correctly
Evaluation 5	Can immobilize beaker’s yeast, perform the experiment on alcohol fermentation, and quantify the glucose almost perfectly	Can immobilize beaker’s yeast, perform the experiment on alcohol fermentation, and quantify the glucose correctly	Can't immobilize beaker’s yeast, perform the experiment on alcohol fermentation, and quantify the glucose correctly

Assigned Department Objectives

学習・教育到達度目標 A-3 （実験を計画・実施し，データの正確な解析に基づいて工学的に考察し，かつ論理的に説明することができる。研究においては与えられた制約の下で必要な援助を得て計画的に研究を進め，期限内にまとめることができる。）

JABEE 1(2)(c) （数学及び自然科学に関する知識とそれらを応用する能力）

JABEE 1(2)(d)(2) （いくつかの工学の基礎的な知識・技術を駆使して実験を計画・遂行し、データを正確に解析し、工学的に考察し、かつ説明・説得する能力）

JABEE 1(2)(h) （与えられた制約の下で計画的に仕事を進め、まとめる能力）

ディプロマポリシー 1 （物質 化学の中心分野である有機化学，無機化学，分析化学，物理化学，生物化学の知識・技術を身につけ，それらを応用した物質創成や技術の開発ができる。）

ディプロマポリシー 2 （課題発見能力，課題解決能力，プレゼンテーション能力を持ち，他者と協働できる。）

ディプロマポリシー 3 （豊かな教養と倫理観を身につけ，社会に貢献できる。）

Teaching Method

Outline:

The purpose of this subject is to learn contents in biochemistry through experiments and to be able to operate measurement instruments. The themes of the experiment are as follows; “Lowry method”, “Enzyme kinetics with β-galactosidase”, “Determination of pKa of amino acids”, and “Alcohol fermentation and
quantification of glucose”

Style:

1) Lecture and experiments
2) Themes are as follows (type A and B)
A) Antibacterial activity test by paper disk method: all at once-experiment
B) Lowry method, enzyme kinetics with β-galactosidase, determination of pKa of amino acids, alcohol fermentation and quantification of glucose: Two experiments for all themes by 4 groups of 8 or 9 students.

Notice:

Failure is no problem, and failure teaches success.
If you fail, try an experiment again in no time.

Course Plan

			Theme	Goals
1st Semester
	1st Quarter
		1st	Explanation of experiments	Can understand the explanation of antibacterial activity test by paper disk method
		2nd	Experiment on antibacterial activity test (1)	Can perform antibacterial activity test by paper disk method
		3rd	Experiment on antibacterial activity test (2)	Can perform antibacterial activity test by paper disk method
		4th	Experiment on antibacterial activity test (3)	Can assess the results of antibacterial activity test by paper disk method
		5th	Lecture (1)	Can understand the explanation of two themes; “Lowry method” and “alcohol fermentation and quantification of glucose”
		6th	Lecture (2)	Can understand the explanation of two themes; “enzyme kinetics with β-galactosidase” and “determination of pKa of amino acids”
		7th	Experiment (1st round of 1st turn)	Can perform appropriate experiment operation and achieve accurate results
		8th	Midterm exam	Midterm exam on antibacterial activity test by paper disk method
	2nd Quarter
		9th	Experiment (2nd round of 1st turn)	Can perform appropriate experiment operation and achieve accurate results
		10th	Experiment (1st round of 2nd turn)	Can perform appropriate experiment operation and achieve accurate results
		11th	Experiment (2nd round of 2nd turn)	Can perform appropriate experiment operation and achieve accurate results
		12th	Experiment (1st round of 3rd turn)	Can perform appropriate experiment operation and achieve accurate results
		13th	Experiment (2nd round of 3rd turn)	Can perform appropriate experiment operation and achieve accurate results
		14th	Experiment (1st round of 4th turn)	Can perform appropriate experiment operation and achieve accurate results
		15th	Experiment (2nd round of 4th turn)	Can perform appropriate experiment operation and achieve accurate results
		16th	Questionnaire	Summarize the study content and confirm grades

Evaluation Method and Weight (%)

	Examination	Presentation	Mutual Evaluations between students	Behavior	Portfolio	Other	Total
Subtotal	20	0	0	0	0	80	100
Basic Ability	0	0	0	0	0	0	0
Technical Ability	20	0	0	0	0	80	100
Interdisciplinary Ability	0	0	0	0	0	0	0